Factors affecting the cryopreservation of oocytes and embryos in buffalo (Bubalus bubalis): A review

Cryopreservation of oocytes and embryos is a supplementary technique for in vivo vitro embryo production. In the last decade, has been improved but did not achieve satisfactory commercial level. Also, results were variable. The pregnancy live birth rates 16 to 52% 5.3 26.9% after implantation vitrified embryos, respectively (from year 1993 2020). final consideration, quality most important factor obtain better cryopreservation. addition antioxidant lipid-depleting agents medium that improves cryogenic tolerance. Additionally, two-step vitrification reliable method Studying epigenetic effects introducing lipidomic, transcriptomic proteomic technologies can help improve this technology.